Lednicky has training and experience in microbiology and molecular biology, various bacteria, fungi and viruses, including influenza and coronaviruses. Research in his aerobiology laboratory includes studies on the mechanisms of respiratory pathogens, assessments of inhalation threats posed by airborne pathogens, vaccine efficacy/safety studies, and the refinement of air-sampling technologies for the detection of airborne pathogens. Recent research includes finding that the Zika virus was present in Haiti several months before the first Zika cases were identified in Brazil, and the discovery of a case of Mayaro virus in a patient in Haiti, the first time the mosquito-borne virus has been detected outside of the Amazon. Since January 2020, a major thrust of his work has been work related to COVID-19, including studying airborne transmission of the SARS-CoV2 virus.
Industry Expertise (2)
Health and Wellness
Areas of Expertise (8)
West Nile Virus
Media Appearances (5)
CDC says surface contact is low cause of COVID-19 spread, updates guidance
WINK News online
“We were wiping packages we were getting from Amazon, leaving them in the corner for one week so the virus would die,” said Professor John Lednicky, an aerovirologist at UF. “Any of that really help anything? No.”
Coronavirus hit Florida earlier than we thought — on a door handle at UF
Tampa Bay Times online
For 19 days, professor John Lednicky and his laboratory took swab samples from a door handle at a busy campus building, and on four of those days they found a strain of influenza. But one day’s samples — from Feb. 21, 2020 — yielded something more notable: The researchers found the same strain of COVID-19 that had been circulating in Washington state at that time.
An interest in bat viruses led to UF Health virologist’s development of coronavirus test
UF Health Newsroom online
University of Florida Health virologist John Lednicky was in the seventh grade, living in the Philippines where his father worked, when he enjoyed a meal of a delicious bat stew. Fruit bat was a local delicacy.
Coronavirus Myth-busting; local expert weighs in on COVID-19
"This is a very profound question and I will explain why they are both correct the CDC information is based on available information and when they calculate the mortality number what they are looking at is the number of deaths over the number of people who have been laboratory confirmed to have the virus," John Lednicky of the Emerging Pathogens Institute said. "So, that fatality rate is probably way too high."
University of Florida online
UF research professor John Lednicky can pull live viruses out of thin air—and grow them. His past decades of inquiry into coronaviruses have positioned him as one of UF’s go-to experts on the newly emerged SARS-CoV-2 pathogen that is spreading globally.
Integration of sample preparation with RNA-Amplification in a hand-held device for airborne virus detectionAnalytica Chimica Acta
Aerosol transmission is one of the three major transmission routes of respiratory viruses. However, the dynamics and significance of the aerosol transmission route are not well understood, partially due to the lack of rapid and efficient tools for on-the-spot detection of airborne viruses. We report a hand-held device that integrates a 3D-printed sample preparation unit with a laminated paper-based RNA amplification unit.
Orthobunyaviruses in the Caribbean: Melao and Oropouche virus infections in school children in Haiti in 2014PLOS Neglected Tropical Diseases
We report the identification of two orthobunyaviruses, Melao virus (MELV) and Oropouche virus (OROV), in plasma specimens from Haitian children with acute febrile illness who presented during outbreaks caused by alpha- and flaviviruses in 2014. Heretofore not described as a human pathogen, MELV was isolated in cell culture from the plasma of five case patients.
Performance of SARS-CoV-2 rapid antigen test compared with real-time RT-PCR in asymptomatic individualsInternational Journal of Infectious Diseases
Screening, testing and contact tracing plays a pivotal role in control of the COVID-19 pandemic. To enable this it is necessary to increase the testing capacity. This study compared a SARS-CoV-2 rapid antigen test (RAT) and RT-PCR in 842 asymptomatic individuals from Tarapacá, Chile. A sensitivity of 69.86%, specificity of 99.61%, PPV of 94.44% and NPP of 97.22% with Ct values (Ct > 27) that were significantly higher among individuals with false-negative RAT were reported.
Organochlorine Pesticide Dieldrin Suppresses Cellular Interferon-related Antiviral Gene ExpressionToxicological Sciences
Organochlorine pesticides (OCPs) are persistent pollutants linked to diverse adverse health outcomes. Environmental exposure to OCPs has been suggested to negatively impact the immune system but their effects on cellular antiviral responses remain unknown. Transcriptomic analysis of N27 rat dopaminergic neuronal cells unexpectedly detected high level expression of genes in the interferon-related anti-viral response pathways including the interferon-induced protein with tetratricopeptide repeats 1 and 2 (Ifit1/2) and the MX Dynamin Like GTPases Mx1 and Mx2.
Genome Sequence of a Yunnan Orbivirus Isolated from a Dead Florida White-Tailed Deer (Odocoileus virginianus)Microbiology Resource Announcements
We report the complete coding sequences of a Yunnan orbivirus isolated from a dead white-tailed deer (Odocoileus virginianus) in Florida in 2019. The prevalence of Yunnan orbivirus and its role in disease among farmed white-tailed deer remain to be determined.