Chamteut Oh
Assistant Professor University of Florida
- Gainesville FL
Chamteut Oh specializes in microbial water quality including environmental DNA/RNA surveillance and disinfection technologies.
Biography
Areas of Expertise
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Articles
Portable, single nucleotide polymorphism-specific duplex assay for virus surveillance in wastewater
Science of the Total EnvironmentOh, et al.
2024-02-20
The Argonaute protein from the archaeon Pyrococcus furiosus (PfAgo) is a DNA-guided nuclease that targets DNA with any sequence. We designed a virus detection assay in which the PfAgo enzyme cleaves the reporter probe, thus generating fluorescent signals when amplicons from a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay contain target sequences.
Improved performance of nucleic acid-based assays for genetically diverse norovirus surveillance
Applied and Environmental MicrobiologyOh, et al.
2023-10-04
Nucleic acid-based assays, such as polymerase chain reaction (PCR), that amplify and detect organism-specific genome sequences are a standard method for infectious disease surveillance. However, challenges arise for virus surveillance because of their genetic diversity. Here, we calculated the variability of nucleotides within the genomes of 10 human viral species in silico and found that endemic viruses exhibit a high percentage of variable nucleotides (e.g., 51.4% for norovirus genogroup II).
A novel approach to concentrate human and animal viruses from wastewater using receptors-conjugated magnetic beads
Water ResearchOh, et al.
2022-04-01
Viruses are present at low concentrations in wastewater; therefore, an effective method for concentrating virus particles is necessary for accurate wastewater-based epidemiology (WBE). We designed a novel approach to concentrate human and animal viruses from wastewater using porcine gastric mucin-conjugated magnetic beads (PGM-MBs). We systematically evaluated the performances of the PGM-MBs method (sensitivity, specificity, and robustness to environmental inhibitors) with six viral species, including Tulane virus (a surrogate for human norovirus), rotavirus, adenovirus, porcine coronavirus (transmissible gastroenteritis virus or TGEV), and two human coronaviruses (NL63 and SARS-CoV-2) in influent wastewater and raw sewage samples.
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