Piyush Jain
Associate Professor University of Florida
- Gainesville FL
Piyush Jain researches the molecular basis of DNA and ways to improve technologies such as CRISPR and Cas.
Biography
Areas of Expertise
Media Appearances
Nobel Prize for chemistry honors exquisitely precise gene-editing technique, CRISPR – a gene engineer explains how it works
The Conversation online
2020-10-07
Researchers have been able to manipulate large chunks of genetic code for almost 50 years. But it is only within the past decade that they have been able to do it with exquisite precision – adding, deleting and substituting single units of the genetic code just as an editor can manipulate a single letter in a document.
Rapid home-based coronavirus tests are coming together in research labs — we’re working on analyzing spit using advanced CRISPR gene editing techniques
The Conversation online
2020-05-20
A desperately needed tool to curb the COVID-19 pandemic is an inexpensive home-based rapid testing kit that can detect the coronavirus without needing to go to the hospital.
Researchers hope CRISPR gene-editing technology can yield rapid at-home COVID-19 test
CBC Radio radio
2020-05-29
Piyush Jain, a biomolecular scientist and assistant professor at the University of Florida, says the technique could be used to detect the RNA — a molecule related to DNA — of SARS-CoV-2, which causes COVID-19, in human saliva.
Social
Articles
Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs
Cell ReportsNguyen, et al.
2024-02-27
We present an exhaustive investigation of 23 Cas12a orthologs, with a focus on their cis- and trans-cleavage activities in combination with noncanonical crRNAs. Through biochemical assays, we observe that some noncanonical crRNA:Cas12a effector complexes outperform their corresponding wild-type crRNA:Cas12a.
Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids
Cell Reports MedicineLong T. Nguyen, et. al
2023-05-08
CRISPR-Cas-based diagnostics have the potential to elevate nucleic acid detection. CRISPR-Cas systems can be combined with a pre-amplification step in a one-pot reaction to simplify the workflow and reduce carryover contamination. Here, we report an engineered Cas12b with improved thermostability that falls within the optimal temperature range (60°C–65°C) of reverse transcription-loop-mediated isothermal amplification (RT-LAMP).
Programmable RNA detection with CRISPR-Cas12a
Nature CommunicationsSantosh R. Rananaware, et. al
2023-01-30
CRISPR is a prominent bioengineering tool and the type V CRISPR-associated protein complex, Cas12a, is widely used in diagnostic platforms due to its innate ability to cleave DNA substrates. Here we demonstrate that Cas12a can also be programmed to directly detect RNA substrates without the need for reverse transcription or strand displacement.
A thermostable Cas12b from Brevibacillus leverages one-pot discrimination of SARS-CoV-2 variants of concern
eBioMedicineLong T. Nguyen, et al.
2022-03-01
Current SARS-CoV-2 detection platforms lack the ability to differentiate among variants of concern (VOCs) in an efficient manner. CRISPR/Cas-based detection systems have the potential to transform the landscape of COVID-19 diagnostics due to their programmability; however, most of these methods are reliant on either a multi-step process involving amplification or elaborate guide RNA designs.
Clinical validation of engineered CRISPR/Cas12a for rapid SARS-CoV-2 detection
Communications MedicineLong T. Nguyen, et al.
2022-01-12
The coronavirus disease caused by SARS-CoV-2 has swept through the globe at an unprecedented rate. CRISPR-based detection technologies have emerged as a rapid and affordable platform that can shape the future of diagnostics.
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
Nature CommunicationsNguyen, et al.
2020-09-30
The CRISPR-Cas12a RNA-guided complexes have tremendous potential for nucleic acid detection but are limited to the picomolar detection limit without an amplification step. Here, we develop a platform with engineered crRNAs and optimized conditions that enabled us to detect various clinically relevant nucleic acid targets with higher sensitivity, achieving a limit of detection in the femtomolar range without any target pre-amplification step.
Media
