Randy Blakely, Ph.D.
Executive Director, FAU Brain Institute & Professor, Biomedical Science Florida Atlantic University
- Boca Raton FL
Randy Blakely is an expert in molecular neuroscience, focusing on how diseases and drugs impact the function of brain synapses and circuits.
Multimedia
Social
Biography
Areas of Expertise
Accomplishments
ulius Axelrod Award
ASPET
Astellas Award
Translational Pharmacology
Zenith Award
Alzheimer’s Association
Distinguished Investigator Award
Brain and Behavioral Research Foundation
MERIT Award
NIMH
Daniel Efron Award
ACNP
Education
Johns Hopkins School of Medicine
Ph.D.
Emory University
B.A.
Selected Media Appearances
Randy Blakely Receives 2022 SfN Science Educator Award
Dana Foundation online
2022-11-14
Each year, the Society for Neuroscience recognizes outstanding neuroscientists who have strongly added to public education and awareness about the field. The Dana Foundation sponsors these awards. This year’s award was presented to Randy Blakely, Ph.D., Florida Atlantic University Stiles-Nicholson Brain Institute.
Rare Human Gene Variant in ADHD, Autism Exposes Fundamental Sex Differences
Neuroscience News online
2022-09-21
The new research published in the journal Molecular Psychiatry and led by Randy Blakely, Ph.D., professor of biomedical science in FAU’s Schmidt College of Medicine and executive director of the FAU Stiles-Nicholson Brain Institute, provides new insight into how sex determines the mechanisms by which distinct synapses monitor and regulate dopamine signaling.
The scientific reason it feels so good to come home after a long day
Ladders online
2020-11-17
“Our data provide clear evidence of a biochemical foundation for the reinforcing properties of home cage return. This simple environmental manipulation can provide a minimally-invasive approach to peel away aspects of reward circuitry connected to natural reinforcers – one that is critical to an animal’s survival,” says senior study author Randy Blakely, Ph.D., executive director of FAU’s Brain Institute and a professor of biomedical science in FAU’s Schmidt College of Medicine, in a release. [...]
Serotonin Link to Autism?
Ivanhoe online
2020-06-05
“The work that we do on the brain focuses on certain brain chemicals,” shared Randy Blakely, PhD, a professor at Florida Atlantic University. [...]
What Is Serotonin and How Does It Affect Your Mood?
Health online
2020-03-04
“Imagine a revolving door that amino acids have to go through to enter the brain,” says Randy Blakely, PhD, professor at the Charles E. Schmidt College of Medicine at Florida Atlantic University and executive director of the FAU Brain Institute. “Only so many can get in at once.” [...]
FAU marks 20 years in Jupiter with groundbreaking for $35M neuroscience facility, new dorms
Palm Beach Post online
2019-11-21
FAU’s Brain Institute, which backs research and education related to neuroscience, has been gearing up for — and attracting faculty to fill — the new space for years, said Randy Blakely, its executive director. [...]
FAU Brain Institute announces ‘Brainy Days 2019,’ a celebration of neuroscience
The Boca Raton Tribune online
2019-02-26
“The ‘Brainy Days’ program represents a focused opportunity for Brain Institute scientists and their trainees to move out of the laboratory and connect with members of our community, young and old, concerning the many exciting developments in brain science and medicine,” said Randy D. Blakely, Ph.D., executive director of FAU’s Brain Institute and a professor of biomedical science in FAU’s Schmidt College of Medicine. [...]
FAU Brain Institute Fellows receive $1.1 million from Florida Department of Health
Eurek Alert online
2018-12-19
"Florida is No. 1 in per capita cases of Alzheimer's disease in the United States," said Randy D. Blakely, Ph.D., director of FAU's Brain Institute. "Members of the FAU Brain Institute are addressing and tackling all aspects of Alzheimer's disease from behavior to biomedicine and engineering. [...]
Scientists pinpoint pathway that impacts features of autism
Science Daily online
2018-10-08
Randy Blakely, Ph.D., senior author, executive director of FAU's Brain Institute, and a professor of biomedical science in FAU's Schmidt College of Medicine, first identified and cloned the human SERT gene about 25 years ago and established the ability of SERT to be blocked by the major antidepressant medications such as Prozac®, Zoloft® and Lexapro®. [...]
Selected Articles
Adrenal serotonin derives from accumulation by the antidepressant-sensitive serotonin transporter
Pharmacological ResearchRandy Blakely et al.
2019
Adrenal chromaffin cells comprise the neuroendocrine arm of the sympathetic nervous system and secrete catecholamines to coordinate the appropriate stress response. Deletion of the serotonin (5-HT) transporter (SERT) gene in mice (SERT−/− mice) or pharmacological block of SERT function in rodents and humans augments this sympathoadrenal stress response (epinephrine secretion). The prevailing assumption is that loss of CNS SERT alters central drive to the peripheral sympathetic nervous system. Adrenal chromaffin cells also prominently express SERT where it might coordinate accumulation of 5-HT for reuse in the autocrine control of stress-evoked catecholamine secretion. To help test this hypothesis, we have generated a novel mouse model with selective excision of SERT in the peripheral sympathetic nervous system (SERTΔTH), generated by crossing floxed SERT mice with tyrosine hydroxylase Cre driver mice. SERT expression, assessed by western blot, was abolished in the adrenal gland but not perturbed in the CNS of SERTΔTH mice. SERT-mediated [3H] 5-HT uptake was unaltered in midbrain, hindbrain, and spinal cord synaptosomes, confirming transporter function was intact in the CNS. Endogenous midbrain and whole blood 5-HT homeostasis was unperturbed in SERTΔTH mice, contrasting with the depleted 5-HT content in SERT−/− mice. Selective SERT excision reduced adrenal gland 5-HT content by ≈ 50% in SERTΔTH mice but had no effect on adrenal catecholamine content. This novel model confirms that SERT expressed in adrenal chromaffin cells is essential for maintaining wild-type levels of 5-HT and provides a powerful tool to help dissect the role of SERT in the sympathetic stress response.
Conditional Knockout of the Serotonin Transporter (SERT) Demonstrates Its Role in Accumulating and Maintaining 5-HT Homeostasis in the Sympathoadrenal System
Biophysical JournalRandy Blakely et al.
2019
Adrenal chromaffin cells (ACCs) comprise the neuroendocrine arm of the sympathetic nervous system and secrete catecholamines to coordinate the physiological response to stress. Although SERT is prominently expressed in ACCs, its role there has remained unclear. Our data show that SERT interacts with 5-HT 1A receptors to modulate catecholamine exocytosis from isolated ACCs and we propose that adrenal SERT coordinates accumulation of 5-HT for reuse in the autocrine control of catecholamine secretion. Consistent with this, constitutive knockout of SERT (SERT −/− mice) augments stress-evoked epinephrine secretion in vivo. However, the global nature of the knockout makes it difficult to determine if SERT might be acting in the CNS and / or ACCs. Furthermore, lack of SERT in platelets disrupts delivery of gut-derived 5-HT to the adrenal gland (and other tissues) via the bloodstream. To address these confounding factors, we generated a novel transgenic mouse model with selective excision of SERT in the peripheral sympathetic nervous system (SERT ΔTH) by crossing floxed SERT mice with tyrosine hydroxylase Cre driver mice. SERT expression, assessed by western blot, was abolished in the adrenal gland but not perturbed in the CNS of SERT ΔTH mice. SERT-mediated [ 3H] 5-HT uptake was unaltered in midbrain, hindbrain, and spinal cord synaptosomes, confirming transporter function was intact in the CNS. Endogenous midbrain and whole blood 5-HT homeostasis was unperturbed in SERT ΔTH mice, contrasting with the adrenal gland, which showed a ≈ 50 % reduction of 5-HT content but with no change in catecholamine content. This novel model confirms that SERT expressed in adrenal chromaffin cells is essential for maintaining 5-HT homeostasis and provides a powerful tool to help dissect the role of adrenal SERT in the sympathetic stress response.
Human Serotonin Transporter Coding Variation Establishes Conformational Bias with Functional Consequences
ACS Chemical NeuroscienceRandy Blakely
2019
The antidepressant-sensitive serotonin (5-HT) transporter (SERT) dictates rapid, high-affinity clearance of the neurotransmitter in both the brain and periphery. In a study of families with multiple individuals diagnosed with autism spectrum disorder (ASD), we previously identified several, rare, missense coding variants that impart elevated 5-HT transport activity, relative to wild-type SERT, upon heterologous expression as well as in ASD subject lymphoblasts. The most common of these variants, SERT Ala56, located in the transporter’s cytosolic N-terminus, has been found to confer in transgenic mice hyperserotonemia, an ASD-associated biochemical trait, an elevated brain 5-HT clearance rate, and ASD-aligned behavioral changes. Hyperfunction of SERT Ala56 has been ascribed to a change in 5-HT KM, though the physical basis of this change has yet to be elucidated. Through assessments of fluorescence resonance energy transfer (FRET) between cytosolic N- and C-termini, sensitivity to methanethiosulfonates, and capacity for N-terminal tryptic digestion, we obtain evidence for mutation-induced conformational changes that support an open-outward 5-HT binding conformation in vitro and in vivo. Aspects of these findings were also evident with another naturally occurring C-terminal SERT coding variant identified in our ASD study, Asn605. We conclude that biased conformations of surface resident transporters that can impact transporter function and regulation are an unappreciated consequence of heritable and disease-associated SERT coding variation.
Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities
ImmunityRandy Blakely et al.
2019
Interleukin-1 (IL-1) signaling is important for multiple potentially pathogenic processes in the central nervous system (CNS), but the cell-type-specific roles of IL-1 signaling are unclear. We used a genetic knockin reporter system in mice to track and reciprocally delete or express IL-1 receptor 1 (IL-1R1) in specific cell types, including endothelial cells, ventricular cells, peripheral myeloid cells, microglia, astrocytes, and neurons. We found that endothelial IL-1R1 was necessary and sufficient for mediating sickness behavior and drove leukocyte recruitment to the CNS and impaired neurogenesis, whereas ventricular IL-1R1 was critical for monocyte recruitment to the CNS. Although microglia did not express IL-1R1, IL-1 stimulation of endothelial cells led to the induction of IL-1 in microglia. Together, these findings describe the structure and functions of the brain’s IL-1R1-expressing system and lay a foundation for the dissection and identification of IL-1R1 signaling pathways in the pathogenesis of CNS diseases.
Serotonin transporter inhibition and 5-HT2C receptor activation drive loss of cocaine-induced locomotor activation in DAT Val559 mice
NeuropsychopharmacologyRandy Blakely et al.
2018
Dopamine (DA) signaling dysfunction is believed to contribute to multiple neuropsychiatric disorders including attention-deficit/hyperactivity disorder (ADHD). The rare DA transporter (DAT) coding substitution Ala559Val found in subjects with ADHD, bipolar disorder and autism, promotes anomalous DA efflux in vitro and, in DAT Val559 mice, leads to increased reactivity to imminent handling, waiting impulsivity, and enhanced motivation for reward. Here, we report that, in contrast to amphetamine and methylphenidate, which induce significant locomotor activation, cocaine administration to these mice elicits no locomotor effects, despite retention of conditioned place preference (CPP). Additionally, cocaine fails to elevate extracellular DA. Given that amphetamine and methylphenidate, unlike cocaine, lack high-affinity interactions with the serotonin (5-HT) transporter (SERT), we hypothesized that the lack of cocaine-induced hyperlocomotion in DAT Val559 mice arises from SERT blockade and augmented 5-HT signaling relative to cocaine actions on wildtype animals. Consistent with this idea, the SERT blocker fluoxetine abolished methylphenidate-induced locomotor activity in DAT Val559 mice, mimicking the effects seen with cocaine. Additionally, a cocaine analog (RTI-113) with greater selectivity for DAT over SERT retains locomotor activation in DAT Val559 mice. Furthermore, genetic elimination of high-affinity cocaine interactions at SERT in DAT Val559 mice, or specific inhibition of 5-HT2C receptors in these animals, restored cocaine-induced locomotion, but did not restore cocaine-induced elevations of extracellular DA. Our findings reveal a significant serotonergic plasticity arising in the DAT Val559 model that involves enhanced 5-HT2C signaling, acting independently of striatal DA release, capable of suppressing the activity of cocaine-sensitive motor circuits.
