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Citizen Science project set to explore the microbiome of kitchen chopping boards
Researchers in the College of Health and Life Sciences at Aston University have been awarded funds to explore the microbiome of the kitchen chopping board with the help of ‘citizen scientists’. The grant is from UK Research and Innovation (UKRI) and the Food Standards Agency (FSA) as part of a larger project to investigate food standard challenges. The new citizen science project plans to recruit participants from underrepresented communities in the West Midlands to investigate levels of foodborne bacteria in the home and produce educational materials for their communities. Citizen science projects put the public at the heart of the research process. Rather than being the subjects of the research, citizens are actively involved in collecting and analysing data, and even deciding what questions they want to ask and co-developing the approaches with researchers citizen science also gives participants the opportunity to directly contribute to scientific research and influence policy. The research team in the School of Biosciences will recruit citizen scientists through its students, who will act as ambassadors in their own households and communities. The team will create methods for sampling bacteria from chopping boards and gather their observations with their team of citizen scientists and ambassadors. This will enable the researchers to identify the bacteria present and determine their antimicrobial resistance (AMR) profiles, providing opportunities for ambassadors and citizens to perform lab research. The researchers, alongside their ambassadors and citizens, will then co-design and disseminate educational materials on food hygiene tailored to their target communities and based on the findings of the study. Dr Alan Goddard, senior lecturer in the School of Biosciences and project lead, said: "Many foodborne infections begin in the home, often through poor hygiene where chopping boards provide an opportunity for raw foods to cross-contaminate. “This is why this project is an exciting opportunity to work with our students and communities to investigate a microbiological problem that causes significant disease every year. By working with the public, we get privileged access to authentic environments and can ensure our solutions are appropriate." At present, around 40 per cent of outbreaks of foodborne infections in Europe occur at home, with approximately 2.4 million cases of food poisoning occurring in the UK annually, leading to 180 deaths. A common source of such infections is poor food hygiene, with chopping boards, where raw foods may cross-contaminate, playing a key role in the infection chain. Misunderstandings, or poor food hygiene, may therefore contribute a significant disease burden. Professor Anthony Hilton, executive dean of the College of Health and Life Sciences said: “This exciting project brings together the expertise of University researchers with the natural inquisitiveness of members of the public to co-develop and undertake a research project which has the potential for real impact in reducing the burden of foodborne disease in the home.” The FSA and UKRI have awarded a total of £200,000 to fund six projects in order to bring the public and researchers together to investigate food standards challenges. All six projects include exploring the bacteria on home grown produce, parents testing the safety of baby formula, and people with food hypersensitivities analysing the allergens in food bought online. The citizen science projects are all linked to the FSA’s Areas of Research Interest themes, covering issues such as antimicrobial resistance (AMR), food hypersensitivity and food safety and hygiene in the home. The funding was delivered in collaboration with the Biotechnology and Biological Sciences Research Council (BBSRC) and the Economic and Social research Council (ESRC), both part of UKRI. It is part of a wider effort to coordinate activities and develop a joined-up approach to tackle the challenges of maintaining safe food in the UK. Professor Robin May, Chief Scientific Advisor for the FSA said: "I’m delighted that the FSA is supporting these exciting citizen science projects across the country. In addition to delivering invaluable data, these projects will allow the communities we serve to help build the evidence on which policy decisions are made. We are committed to using science and evidence to tackle the latest food-related issues and citizen science is a fantastic way of doing this." The citizen science project investigating the microbiome of the kitchen chopping at Aston University will start in January 2022, concluding in July 2022.
Toolkit is easily incorporated into any laboratory science course Wilmington, Del., April 1, 2021 – St. Georges Technical High School in southern New Castle County, Delaware is the first high school in the United States to use ChristianaCare Gene Editing Institute’s innovative CRISPR in a Box Educational Toolkit™ in a science class. CRISPR in a Box brings to life the much-heralded CRISPR gene editing technology – the “genetic scissors” that allow scientists to edit DNA. The toolkit is designed for educational sessions in secondary and post-secondary schools and is suitable for remote learning. “Gene editing is the future of medicine,” said Eric Kmiec, Ph.D., director of ChristianaCare’s Gene Editing Institute. “Our partnership with the Delaware Department of Education will help cultivate the next generation of genetic scientists and enhance Delaware’s position as a leader in the biosciences.” “We are thrilled that students at St. Georges Technical High School will be the first In the United States to experience a live demonstration of CRISPR gene editing using our Innovative CRISPR in a Box educational toolkit,” said Siobhan Hawthorne, Education and Community Outreach leader at ChristianaCare’s Gene Editing Institute. “This toolkit will provide STEM students with a visual understanding of how the exciting CRISPR technology can unlock medical treatments to improve lives.” Delaware Secretary of Education Susan Bunting praised her department's partnership with ChristianaCare's Gene Editing Institute to develop the “Seeds of STEM” course that teaches high school students about gene editing. “Gene editing approaches diseases in new ways and will have significant impact in the health care and agriscience fields,” Bunting said. “This is a great example of an industry and education partnership investing in youth by providing hands-on knowledge and skills around emerging technology.” “We are so fortunate that ChristianaCare’s Gene Editing Institute reached out to our program to plan a high school ‘first’ opportunity with this new CRISPR experiment,” said Danya Espadas, one of the St. Georges biotech teachers. “Giving students the chance to use a cutting-edge, 21st century tool for medicine in their own high school lab – to have that technology at their fingertips – transcends what they see in a textbook or a video. By being able to do it themselves, it makes it real for them.” Espada said the experiment focuses on editing a gene of a non-infectious E.coli bacteria to become resistant to an antibiotic, thereby allowing researchers to create a new class of antibiotics that cannot be overcome by bacteria that are gene resistant. “We’re talking about eventually saving lives, here,” she said. “What can be more important than that?” The tools in CRISPR in a Box have been designed based on the pioneering discoveries of the Gene Editing Institute that are currently being used to explore next-generation medical therapies and diagnostics for diseases, including lung cancer and sickle-cell anemia. Component items in the toolkit include the CRISPR/Cas complex, a target DNA molecule, a mammalian cell free extract and a synthetic DNA molecule. All materials in the kit are safe, synthetic materials. There are no live cultures or viruses involved. The kit is meant to provide a hands-on demonstration of CRISPR’s capabilities, and not allow for manipulations of living organisms. “The kit is easy and fun to use,” said Kristen Pisarcik, research assistant at the Gene Editing Institute who has taught students at Delaware Technical Community College which first used the toolkit. “In a short period of time students will reliably and successfully complete the laboratory activity and be able to see the results of gene editing,” she said. Since the foundations of the kit touch upon key themes in biology, it can be readily incorporated into practically any science or biology course with a laboratory component, “One of the beauties of CRISPR in a Box is that there is no need to purchase specialized equipment. If a teaching lab can support bacterial cultivation, it can perform the in vitro gene editing lab activity,” Pisarcik said. CRISPR in a Box is the evolution of a partnership between the Gene Editing Institute, Delaware Technical Community College and Rockland Immunochemicals that began in 2017 with a National Science Foundation grant to develop the first-ever gene editing curriculum for community college students. Video and photo collection of first class in U.S. to use CRISPR in a Box™ educational gene editing toolkit. About ChristianaCare’s Gene Editing Institute The Gene Editing Institute, a worldwide leader in CRISPR gene editing technology and the only institute of its kind based within a community health care system, takes a patient-first approach in all its research to improve the lives of people with life-threatening disease. Since 2015, researchers at the Gene Editing Institute have been involved in several ground-breaking firsts in the field, including the development of the first CRISPR gene editing tool to allow DNA repairs outside the human cell which will rapidly speed therapies to patients and a unique version of CRISPR called EXACT that reduces the number of off-target edits to other areas of the genome, which is vital for further research and patient applications. Its researchers are currently developing a patient trial for lung cancer using CRISPR and employing the technology to combat the COVID-19 pandemic. About the biotech program St. Georges Technical High School The Biotech career program of study at St. Georges Technical High School is the first such program offered in a Delaware high school. With two teachers and approximately 100 students in grades 10-12, the program presents advanced content in biology and chemistry with opportunities for students to learn basic laboratory techniques and procedures and to maintain and operate common instruments and equipment used in a biotechnology laboratory. St. Georges is a comprehensive career and technical high school with 1,100 students who study in one of 16 different career pathways.
Rensselaer Team Seeks Alternative Approach to Controlling Viruses
As researchers worldwide scramble to formulate a vaccine to combat COVID-19, a team at Rensselaer Polytechnic Institute is pursuing a potentially powerful solution to pandemic control: a viral trap that is easily adapted to different classes of viruses, enabling a “plug-and-play” approach to virus detection and antiviral activity. Jonathan Dordick, an endowed professor of chemical and biological engineering at Rensselaer, and Robert Linhardt, an endowed professor of chemistry and chemical biology, said the team is exploring how their work — in the areas of viral detection, therapy, and inhibition — could be used against COVID-19 and other viruses in the future. Their team views such innovative approaches as a vital hedge against the growing threat of global pandemics. The viral trap works by mimicking the latch points on a human cell that a virus must bind to before infecting a person by disgorging its genetic instructions into the cell. In research on the Dengue virus with Xing Wang, now a professor of chemistry at the University of Illinois, recently published in Nature Chemistry, the team folded a snippet of DNA into a five-pointed star, and attached decoy latch points that align perfectly with the virus’ own molecular grappling hooks. The result was the world’s most sensitive test for Dengue, and a novel means of capturing and ultimately killing the virus. In previous research, the team demonstrated the same approach for Influenza A, and it can likely be expanded to other viruses like COVID-19. In another approach, Dordick demonstrated how enzymes incorporated into paint, can form a catalytic coating capable of killing the Influenza A Virus. The research, published in Applied Microbiology and Biotechnology, suggests enzyme systems could further be incorporated into swabs, wipes, or coatings, to target and kill various viruses, including COVID-19.
Researchers at Rensselaer Can Now 3D Print Skin With Working Blood Vessels
Researchers at Rensselaer Polytechnic Institute have developed a way to 3D print living skin, complete with blood vessels. The advancement, published online in Tissue Engineering Part A, is a significant step toward creating grafts that are more like the skin our bodies produce naturally. “Right now, whatever is available as a clinical product is more like a fancy Band-Aid,” said Pankaj Karande, an associate professor of chemical and biological engineering and member of the Center for Biotechnology and Interdisciplinary Studies (CBIS), who led this research at Rensselaer. “It provides some accelerated wound healing, but eventually it just falls off; it never really integrates with the host cells.” A significant barrier to that integration has been the absence of a functioning vascular system in the skin grafts. Karande has been working on this challenge for several years, previously publishing one of the first papers showing that researchers could take two types of living human cells, make them into “bio-inks,” and print them into a skin-like structure. Since then, he and his team have been working with researchers from Yale School of Medicine to incorporate vasculature. In this paper, the researchers show that if they add key elements — including human endothelial cells, which line the inside of blood vessels, and human pericyte cells, which wrap around the endothelial cells — with animal collagen and other structural cells typically found in a skin graft, the cells start communicating and forming a biologically relevant vascular structure within the span of a few weeks. “As engineers working to recreate biology, we’ve always appreciated and been aware of the fact that biology is far more complex than the simple systems we make in the lab,” Karande said. “We were pleasantly surprised to find that, once we start approaching that complexity, biology takes over and starts getting closer and closer to what exists in nature.” You can watch Pankaj Karande, associate professor of chemical and biological engineering, explain this research here: Pankaj Karande is an associate professor of chemical and biological engineering and member of the Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer. He is available to speak with media regarding this latest development – simply click on his icon to arrange an interview.
A Lego-Like Approach to Improve Nature’s Own Ability to Kill Dangerous Bacteria
The Centers for Disease Control and Prevention considers antibiotic resistance one of the most urgent public health threats, one that affects communities worldwide. The ramifications of bacteria’s ability to become resistant to antibiotics can be seen in hospitals, public places, our food supply, and our water. In their search for solutions, researchers at Rensselaer Polytechnic Institute have been looking to nature. In a paper recently published in Biomacromolecules, the team demonstrated how it could improve upon the ability of nature’s exquisitely selective collection of antimicrobial enzymes to attack bacteria in a way that’s much less likely to cause bacterial resistance. “The idea is that we could take nature’s approach and just make it better,” said Jonathan Dordick, a chaired professor of chemical and biological engineering and a member of the Center for Biotechnology and Interdisciplinary Studies (CBIS). In order for bacteria to grow and live, they naturally produce autolysin enzymes that can break down their own cell walls, allowing those cells to divide and multiply. In attacking one another, bacteria take advantage of a similar process, using an antibacterial protein known as a bacteriocin to kill a bacterium. Bacteria can also be attacked by bacteriophages, which are viruses that infect bacteria. They produce phage endolysin enzymes, which attack the bacterial cell from the inside. All three types of enzymes are broadly known as cell lytic enzymes, as they catalyze the breakdown of the bacterial cell wall. “It’s very difficult for bacteria to become resistant to the action of these enzymes,” Dordick said. “For example, if they became resistant to an autolysin, they wouldn’t divide.” Like building blocks, most cell lytic enzymes are modular. They’re made up of one binding domain which attaches to the cell wall, and a catalytic domain that breaks holes in the cell wall — effectively destroying the targeted bacteria. “The idea was: Could we use a Lego-like approach here? Could we take a binding domain from one enzyme and can we mix it with a binding domain or catalytic domain of another one?” Dordick said. The issue of antibiotic resistant bacteria and disease is a serious one and of great concern to the medical community. If you’re a journalist covering this topic or are looking to know more about the ongoing research into this field – let our experts help. Jonathan S. Dordick is the Howard P. Isermann Professor of Chemical and Biological Engineering at Rensselaer Polytechnic Institute where he is also the Senior Advisor to the President for Strategic Initiatives. Dr. Dordick is available to speak with media regarding this topic - simply click on his icon to arrange an interview.
Keratoconus – ever heard of it? It’s an eye condition where genetics and environmental factors like ultraviolet light and vigorous eye rubbing conspire to make the usual curvature of the cornea more pointy, leaving us with double vision and nearsighted. National Basketball Association and Golden State Warriors star Stephen Curry helped make keratoconus, which affects an estimated 1 in 2,000, a more visible eye condition this April. A $2.1 million grant from the National Eye Institute is now helping Dr. Yutao Liu, vision scientist and human geneticist, learn more about keratoconus’ causes and identify points to better diagnose, treat and possibly prevent the progressive disease that typically starts in our teens. “We want to help patients better understand what is happening to their vision by better understanding how keratoconus happens, and give physicians better points to intervene,” says the scientist in the Department of Cellular Biology and Anatomy at the Medical College of Georgia and James and Jean Culver Vision Discovery Institute at Augusta University. For Curry, his solution was simple – treatment with corrective contact lenses. But keratoconus does progress and some who suffer may eventually require a corneal transplant surgery or corneal collagen cross-linking as treatment. Keratoconus is a fascinating disease and the research by experts at Augusta University will be groundbreaking. Do you need to know more? That’s where we can help. Dr. Yutao Liu is an associate professor of Cellular Biology and Anatomy with the Center for Biotechnology and Genomic Medicine at Augusta University. Dr. Liu and is available to speak with media regarding this rare disease - simply click on his icon to arrange an interview.
